Digestion and Absorption Exam Practice Test Questions and Answers

150 Questions and Answers

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A thorough understanding of digestion and nutrient absorption is essential for students in health sciences, nutrition, medicine, and biology. This Digestion and Absorption Exam Practice Test Questions and Answers resource is designed to help learners master the physiological and biochemical processes that fuel the human body, making it an ideal tool for academic exam preparation and clinical understanding.

This comprehensive practice test covers all key stages of digestion—from mechanical and chemical breakdown in the mouth and stomach to enzyme action in the small intestine and nutrient absorption across intestinal walls. Topics include digestive enzymes, gastric secretions, bile function, pancreatic activity, peristalsis, intestinal transport mechanisms, and the roles of the liver and gallbladder in digestion.

You’ll be tested on the digestion and absorption of carbohydrates, proteins, fats, vitamins, and minerals, as well as on associated hormonal regulation, such as the roles of gastrin, secretin, and cholecystokinin (CCK). The questions also explore clinical conditions like malabsorption syndromes, enzyme deficiencies, lactose intolerance, and nutrient transport disorders, reinforcing your ability to apply core concepts to real-world scenarios.

Each question is formatted to reflect exam-style structure, challenging your ability to analyze, reason, and recall with accuracy. Whether you’re preparing for nursing school exams, medical entrance tests, physiology finals, or nutrition certification, this test provides the foundation you need for confident performance.

The Digestion and Absorption Exam Practice Test Questions and Answers tool supports both independent study and classroom review. It helps identify knowledge gaps, reinforces key physiological mechanisms, and ensures a deeper understanding of how the body processes food for energy, growth, and repair.

With content based on current academic standards and designed for practical relevance, this resource is a must-have for students and professionals seeking excellence in digestive physiology and clinical nutrition.

Sample Questions and Answers

Which of the following enzymes is responsible for breaking down proteins in the stomach?
A. Amylase
B. Pepsin
C. Lipase
D. Sucrase
Answer: B

  1. What is the main function of bile in digestion?
    A. Emulsify fats
    B. Neutralize stomach acids
    C. Digest carbohydrates
    D. Absorb vitamins
    Answer: A
  2. Which of the following structures prevent the reflux of food from the stomach back into the esophagus?
    A. Cardiac sphincter
    B. Pyloric sphincter
    C. Ileocecal valve
    D. Anal sphincter
    Answer: A
  3. Which of the following is absorbed by the villi in the small intestine?
    A. Carbohydrates
    B. Proteins
    C. Fats
    D. All of the above
    Answer: D
  4. What substance neutralizes stomach acid in the duodenum?
    A. Bile
    B. Pancreatic enzymes
    C. Bicarbonate ions
    D. Insulin
    Answer: C
  5. Which enzyme is responsible for breaking down starch into maltose?
    A. Amylase
    B. Lipase
    C. Maltase
    D. Sucrase
    Answer: A
  6. What is the function of the large intestine?
    A. Digestion of proteins
    B. Absorption of nutrients
    C. Absorption of water and electrolytes
    D. Secretion of digestive enzymes
    Answer: C
  7. Which of the following structures increases the surface area of the small intestine to aid in absorption?
    A. Plicae circulares
    B. Villi
    C. Microvilli
    D. All of the above
    Answer: D
  8. What is the role of cholecystokinin (CCK) in digestion?
    A. Stimulates gastric acid secretion
    B. Stimulates bile release from the gallbladder
    C. Stimulates insulin release
    D. Stimulates the release of gastric enzymes
    Answer: B
  9. Where does the majority of nutrient absorption occur?
    A. Stomach
    B. Large intestine
    C. Small intestine
    D. Esophagus
    Answer: C
  10. What type of macromolecule is broken down by lipase?
    A. Proteins
    B. Carbohydrates
    C. Fats
    D. Nucleic acids
    Answer: C
  11. Which of the following organs produces insulin and digestive enzymes?
    A. Liver
    B. Gallbladder
    C. Pancreas
    D. Small intestine
    Answer: C
  12. What substance does the liver produce to aid in the digestion of fats?
    A. Insulin
    B. Bile
    C. Amylase
    D. Pepsin
    Answer: B
  13. Which digestive enzyme is responsible for breaking down lactose?
    A. Amylase
    B. Lactase
    C. Maltase
    D. Pepsin
    Answer: B
  14. Which digestive enzyme is responsible for breaking down triglycerides into fatty acids and glycerol?
    A. Amylase
    B. Trypsin
    C. Lipase
    D. Pepsin
    Answer: C
  15. What is the role of the stomach’s mucus lining?
    A. To absorb nutrients
    B. To break down proteins
    C. To protect the stomach from digestive acids
    D. To neutralize stomach acid
    Answer: C
  16. What is the primary function of the duodenum?
    A. Absorption of nutrients
    B. Breakdown of carbohydrates
    C. Digestion and neutralization of stomach acid
    D. Storage of bile
    Answer: C
  17. Which of the following is a function of the pancreas in digestion?
    A. Secretion of bile
    B. Secretion of digestive enzymes
    C. Absorption of nutrients
    D. Breakdown of starch
    Answer: B
  18. Which digestive enzyme breaks down protein molecules into smaller peptides?
    A. Amylase
    B. Lipase
    C. Pepsin
    D. Sucrase
    Answer: C
  19. Which of the following is the function of the pyloric sphincter?
    A. To prevent food from entering the stomach
    B. To regulate the release of food from the stomach into the small intestine
    C. To allow bile to enter the stomach
    D. To prevent reflux from the small intestine to the stomach
    Answer: B
  20. Which of the following is the primary function of the pancreas in digestion?
    A. Secretes bile into the duodenum
    B. Secretes digestive enzymes and bicarbonate
    C. Absorbs nutrients from the small intestine
    D. Stores digestive enzymes
    Answer: B
  21. What is the function of the ileocecal valve?
    A. To prevent food from entering the small intestine
    B. To regulate the passage of food from the stomach into the small intestine
    C. To control the flow of food from the small intestine to the large intestine
    D. To secrete digestive enzymes
    Answer: C
  22. Which type of digestion involves the breakdown of food by enzymes and acids?
    A. Chemical digestion
    B. Mechanical digestion
    C. Absorption
    D. Propulsion
    Answer: A
  23. Which of the following cells produce hydrochloric acid in the stomach?
    A. Chief cells
    B. Parietal cells
    C. Mucous cells
    D. Enteroendocrine cells
    Answer: B
  24. What is the function of the gallbladder in digestion?
    A. To store and concentrate bile
    B. To secrete digestive enzymes
    C. To absorb nutrients
    D. To store glucose
    Answer: A
  25. What is the main function of the enzyme amylase in digestion?
    A. To break down fats
    B. To break down proteins
    C. To break down carbohydrates
    D. To break down nucleic acids
    Answer: C
  26. What is absorbed in the colon (large intestine)?
    A. Nutrients
    B. Water and electrolytes
    C. Carbohydrates
    D. Proteins
    Answer: B
  27. Which part of the small intestine is the first to receive chyme from the stomach?
    A. Jejunum
    B. Ileum
    C. Duodenum
    D. Cecum
    Answer: C
  28. Which of the following is true about the absorption of fats?
    A. Fats are absorbed directly into the bloodstream.
    B. Fats are absorbed into lymphatic vessels.
    C. Fats are absorbed in the large intestine.
    D. Fats are absorbed in the stomach.
    Answer: B
  29. Which of the following enzymes breaks down proteins into smaller peptides in the stomach?
    A. Amylase
    B. Pepsin
    C. Lipase
    D. Trypsin
    Answer: B

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